Phytoplankton Sampling

​Phytoplankton samples were collected on March 20, 2024 at Bullock Reach and analyzed in the laboratory shortly after collection. It was partly sunny, breeze and brisk on Narragansett Bay. The qualitative tow net sample had a long filtration time, and the filtrate was a dark brown color. Some sediment and jellyfish were found in the sample. 

The analysis of the quantitative whole water sample revealed a total of 3,536,000 cells per Liter. The micro flagellates were found at 20,000 cells/L. The most predominant phytoplankton genus was Thalassiosira spp. at 1,888,000 cells/L. Other representative genera include Skeletonema spp. and Leptocylindrus spp.​ Thalassiosira spp. (today’s featured micrograph, 200x phase contrast) is a type of ​​phytoplankton belonging to the centric diatom group. This photosynthetic species grows primarily in marine waters. Some species within the genus are found in estuaries and aquatic systems that have been impacted by human activities. They can be identified by their coin-like shape.

Phytoplankton samples were collected on March 6, 2024 at Bullock Reach and analyzed in the laboratory shortly after collection. It was cloudy and mild on Narragansett Bay with an outgoing tide. The qualitative tow net sample had a moderate filter time, and the filtrate was a golden brown color.

The analysis of the quantitative whole water sample revealed a total of 24,820,000 cells per Liter. The micro flagellates were found at 40,000 cells/L. The most predominant phytoplankton genus was Leptocylindrus spp. at 3,840,000 cells/L. Other representative genera include Skeletonema spp. and Chaetoceros spp. Thalassionema nitzschioides (today’s featured micrograph, 200x phase contrast) ​​is a type of phytoplankton belonging to the pennate diatom group. This photosynthetic species has small and numerous chloroplasts which give it a yellow brown color. The cells are usually found in star-shaped or in zigzagged chains. They occur in waters with salinity between 12-38 ppt and 15°C.

​Phytoplankton samples were collected on December 13, 2023 at Bullock’s Reach and analyzed in the laboratory shortly after ​​​​collection. It was calm and brisk on Narragansett Bay with an outgoing tide. The qualitative tow net sample had a moderate filter time, and the filtrate was a light brown color with some particulate matter. The analysis of the quantitative whole water sample revealed a total of 81,000 cells per liter. The micro flagellates were found at 11,000 cells/L. The most predominant phytoplankton genus was ​Skeletonema spp. at 31,000 cells/L. Other representative genera include Leptocylindrus spp. and Guinardia spp. The photo featured this week is a 200x phase-contrast image of Staurastrum, a type of green algae in the desmid family. It gets its name from the Greek ​​​“stauron” which means cross and “astron” which means star. It is made of highly constricted unicells that are radially symmetrical and can be distinguished by cell wall patterns. Staurastrum is a food source for zooplankton in the aquatic food web.​

Phytoplankton samples were collected on November 30, 2023 at Bullock Reach and analyzed in the laboratory shortly after collection. It was sunny and brisk on Narragansett Bay with calm conditions and a southwest wind at less than 10 kts. The qualitative tow net sample had a quick filter time, and the filtrate was a light tan color. The analysis of the quantitative whole water sample revealed a total of 153,000 cells per liter. The microflagellates were found at 52,000 cells/L. The most predominant phytoplankton genus was Skeletonema spp. at 65,000 cells/L. Other representative genera include Chaetoceros spp. and Eucampia spp. Chaetoceros socialis (see image, 200x phase contrast) is a diatomic phytoplankton found in marine and brackish waters. These phytoplankton have a characteristic linear colonial growth habit and their very long, overlapping, spines are thought to minimize sinking rates.​

Phytoplankton whole water sample was collected on November 15, 2023 at Bullock Reach and analyzed in the laboratory shortly after collection. No qualitative tow-net sample was collected this day. It was calm, clear, and very brisk on Narragansett Bay with clear water about 6.2 meters down. Sonde data revealed the surface water temperature was 10.4°C, salinity was 24.8 psu, and chlorophyll a was 0.8 ug/L. The analysis of the quantitative whole water sample revealed a total of 77,000 cells per Liter. The microflagellates were found at 39,000 cells/L. The most predominant phytoplankton genus was Skeletonema spp. at 13,000 cells/L. Other representative genera include Leptocylindrus spp., Chaetoceros spp. and Pseudo-nitzchia spp.

​Phytoplankton samples were collected on November 1, 2023 at Bullock Reach and analyzed in the laboratory shortly after collection. It was cloudy and brisk on Narragansett Bay with a northwest wind at 10 kts. Sonde data revealed the surface water temperature was 13.08°C, salinity was 22.63 psu, and chlorophyll a was 1.06

ug/L. The qualitative tow net sample had a moderate filter time, and the filtrate was a light yellow-brown color. The analysis of the quantitative whole water sample revealed a total of 119,000 cells per liter. The micro flagellates were found at 62,000 cells/L. The most predominant phytoplankton genus was Skeletonema spp. at 46,000 cells/L. Other representative genera include Leptocylindrus spp., Eucampia spp., and Prorocentrum spp. Prorocentrum micans (see image, 200x phase contrast) is a tear-dropped shaped dinoflagellate that is often found in Narragansett Bay during the late summer-autumn time period. This species can form extensive blooms but is  considered harmless.

Phytoplankton samples were collected on October 18, 2023 at Bullock’s Reach and analyzed in the laboratory shortly after collection. It was a cloudy and chilly day on Narragansett Bay. The water was noticeably clear to almost 5 meters depth. Sonde data revealed the surface water temperature was 15.8°C, salinity was 24.9 psu, and chlorophyll a was 7.85 ug/L. The qualitative tow net sample had a medium filter time, and the filtrate was a light brown color. Some comb jellyfish were detected in the sample.

The analysis of the quantitative whole water sample revealed a total of 1,997,000 cells per Liter. The micro flagellates were found at 419,000 cells/L. The most predominant phytoplankton genus was Skeletonema spp. at 1,255,000 cells/L. Other representative genera include Chaetoceros spp., Leptocylindrus spp., and Cerataulina spp.​​ ​This week’s featured image is the diatom Ditylum brightwellia, captured at 200x phase contrast.

 

A phytoplankton sample was collected on October 4, 2023 at Bullock’s Reach and analyzed in the laboratory shortly after collection. The plankton tow-net sample was not collected this week, only the whole-water quantitative sample. Sonde data revealed the surface water temperature was 18.50°C, salinity was 18.15 psu, and chlorophyll a was 4.23 ug/L. 

The whole water sample was analyzed and revealed a total of 676,000 cells per Liter. The micro flagellates were found at 554,000 cells/L. The most predominant phytoplankton genus was Chaetoceros spp.at 78,000 cells/L. Other representative genera include Eucampia spp. (see 400x phase contrast image below), Skeletonema spp., and Gonyaulax spp.

Phytoplankton samples were collected on September 20, 2023 at Bullock Reach and analyzed in the laboratory shortly after collection. Sonde data revealed the surface water temperature was 19.3°C, salinity was 24.5 psu, and chlorophyll a was 12.2 ug/L. The plankton tow net filtrate was a light brown color and had a long filter time. High particulates and comb jelly fish were detected. The whole water sample was analyzed and revealed a total of 76,000 cells per Liter.  The micro flagellates were found at 28,000 cells/L. The most predominant phytoplankton genus was Leptocylindrus spp.at 27,000 cells/L. Other representative genera include Skeletonema spp. and Chaetoceros spp. Today’s featured micrograph shows a centric diatom at 400x phase contrast. 

Phytoplankton samples were collected on September 6, 2023 at Bullock Reach and analyzed in the laboratory shortly after collection. Sonde data revealed the surface water temperature was 23.4°C, salinity was 24.5 psu, and chlorophyll a was 12.2 ug/L. The plankton tow net sample was filterable with a 20 micron mesh. The filtrate was a light brown greenish color and had a long filter time. The tow net sample was analyzed qualitatively for microorganisms using phase contrast microscopy. The whole water sample was analyzed quantitatively under 200x phase contrast microscopy. A Hensen Stempel pipette was used to accurately deliver 1ml of sample to a Sedge-wick Rafter chamber. This analysis revealed a total of 2,856,000 cells per Liter. The micro flagellates were found at 108,000 cells/L. The most predominant phytoplankton genus was Leptocylindrus spp.at 2,196,000 cells/L. Other representative genera include Skeletonema spp., Chaetoceros spp., and Eucampia spp., the subject of the 200x phase contrast micrograph shown below.